What is Lowry method? what is it used for? --- Protein determination

Lowry method

The lowry method is the biochemical quantification of proteins. Basically, it is an estimation of proteins in a solution. The color change will tell us about the concentration of proteins, which we measure by using calorimetric techniques.

This method is the combination of two reactions

  1. Reaction of copper ions with the peptide bond under alkaline conditions
  2. Oxidation of aromatic protein residues

The Lowry method can be used with protein concentrations of 0.01—1.0 mg/mL. Cu+ ions react with the follin—ciocalteu reagent. Follin—ciocalteu reagent is a mixture of phosphotungstic acid and phosphomolybdic acid. Lowry method involves the reduction of Folin reagent and oxidation of protein residues (tryptophan and tyrosine). Cysteine(residue in protein) is also reactive to the reagent. So, it gives some absorbance. Then we measure the concentration of reduced Folin reagent by absorbance at 750nm. The concentration of tryptophan and tyrosine that reduces the Folin reagent, gives us the quantity of protein in the sample.

 

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